Simple Determination of Dimethylsulfide (DMS) and Dimethylsulfoniopropionate (DMSP) Using Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry

نویسندگان

  • TAKUSHI NIKI
  • TAIKI FUJINAGA
  • MARIYO F. WATANABE
  • JUNJI KINOSHITA
چکیده

Solid-phase microextraction (SPME) is a simple, sensitive and less destructive method for the determination of dimethylsulfide (DMS) in seawater. Combined with detection by gas chromatography-mass spectrometry (GC-MS), the method had sufficient sensitivity (minimum detectable concentration of DMS was 0.05 nM), and practical levels of reproducibility (relative standard deviation ≤7%) and linearity (r2 > 0.995) over a wide concentration range (0.5 to 910 nM). The protocol developed was applied to a Sagami Bay water sample to determine concentrations of DMS and DMSP, and in situ DMSP-lyase activity.

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تاریخ انتشار 2004